Effect of AHP Disinfectants on Cage Integrity

Background

Texas Biomed researchers observed accelerated clouding of polysulfone rodent cages in high containment facilities, preventing proper animal observation. This study evaluated how three commonly used disinfectants affect cage integrity when applied before autoclaving.

Key Findings

• Unexpected Results: Contrary to the hypothesis that phenolic solutions would cause the most damage, the hydrogen peroxide-based disinfectant (Peroxigard) proved most detrimental.
• Significant Damage: Cages treated with Peroxigard showed etching after just one autoclave cycle, developed significant cracking after three cycles, and became unusable after six cycles.
• Superior Performance: Phenolic disinfectant (Wexide), 70% ethanol, and control cages showed no significant change in transparency or integrity even after ten autoclave cycles.

Implications

This study highlights the importance of disinfectant selection in high containment animal facilities. Using Peroxigard before autoclaving significantly compromises polysulfone cage integrity, while phenolic and alcohol-based alternatives maintain cage quality through repeated sterilization cycles.

Recommendations

Facilities should avoid using accelerated hydrogen peroxide-based disinfectants (like Peroxigard) on polysulfonecages when autoclaving is required. Phenolic disinfectants or 70% ethanol appear to be safer alternatives for maintaining cage integrity in high containment settings.